Studying the error for HCV amplicon sequencing with the Illumina MiSeq platform
نویسندگان
چکیده
Next Generation Sequencing (NGS) is a well established technology for acquisition of vast amount of nucleic acid sequence data at very low cost and high throughput. We aim to provide solid framework for the accurate determination of the viral haplotypes of Hepatitis C virus (HCV) using the Illumina MiSeq platform. To do that, we studied the error rates of sequences generated with the longest available read kits for the MiSeq-v3 (600), applied to bar-coded HCV amplicon libraries of low complexity.
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تاریخ انتشار 2016